FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell11.351000000950f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell11.351000000950f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222890
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.3216833415586164No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.22163398986046928No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.22073668625779533No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4160.18663914935618467No Hit
GCCCAGGGAGACGTGTGCTCTTCCGATCTGCCCAGGGAGACGTGTGCTCTT2600.1166494683476154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG450.001223489224.9998745
TTCTAGA500.002256049822.5049361
GTACTGG602.4552102E-422.5049361
GTCCAGG654.2172222E-420.7737881
CCTATAG600.006494608218.7499072
CTGCACT851.19896664E-418.5293228
TGCACTG851.19896664E-418.5293229
ATCTTTT26550.018.3049937
GATCTTT26950.018.2002816
TCTTTTT27350.017.6050328
CCTAGGG901.8572471E-417.4999122
TAAAGGG800.001705065416.8749164
CTTTTTT29000.016.6033659
CATCTCC850.002556559215.88227443
CGATCTT34500.015.7173135
ACACATC2051.2951205E-915.36577745
ATCTGAT1108.550452E-414.318117
CCCCATG1150.001194481213.6986561
CTAGACG1150.001196507713.6955823
CCGTGCC1000.00752620213.4999327
Produced by FastQC (version 0.11.2)