FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell10.351000000950de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell10.351000000950de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222533
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.14379889724220676No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGGAA2380.10695042982389129No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAAG550.003928425720.45301241
TCAGGGG550.003928425720.4530124
GATCTTT33600.019.5521096
ATCTTTT33600.019.2842717
TGACGAG600.006497198318.7485945
TCTTTTT34200.018.6828128
CATCTCC851.19959805E-418.52802545
CTTTTTT36000.017.748679
CGATCTT40450.017.6315575
ATCTACG1209.70223E-514.9988777
TCTGTCC900.003743129314.9988768
CAGACGT1251.3734249E-414.3989234
CTGGCCG1000.00752963213.4989899
CTAGACG1200.001650041313.1240173
ACATCTC1200.001650041313.12401744
CACATCT1607.772693E-512.65530245
GGGGTGC2054.7807353E-612.07226716
GGGAAGA2251.0274616E-611.99910244
TGGCACG1506.4170663E-411.99910110
CCGAAAA1350.00398528211.66841427
Produced by FastQC (version 0.11.2)