FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n01_inf_cell1.35100000094fb8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n01_inf_cell1.35100000094fb8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923232
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA14900.1613895532217254No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGG10840.11741360784721501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATCG651.4040779E-831.1511848
CGATCCA300.005148890429.99743744
CGATCTG8200.029.3572485
AACGGTG903.965397E-1027.4976520
TCCGATC21300.027.0399463
CTTCCGA21500.026.910541
TTCCGAT21550.026.8306542
CCGATCT21550.026.7262554
GATCGGA2800.025.728811
TCGAGCC851.8943683E-723.82149312
CGATCTC5200.023.3633885
TCGGAAG3200.023.2011433
CCGGGTA1700.022.4980776
TATCGAG903.2882053E-722.49807710
TCTTCCG9350.022.3777665
CGGCTCT955.5223245E-721.31858631
CGGTACC955.5338387E-721.31396945
CGATCTA2850.021.3139695
CGGGTAC2450.021.1206447
CGATCTT7600.021.0179425
Produced by FastQC (version 0.11.2)