FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell96.35200000095b9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell96.35200000095b9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737773
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA29130.10640034802008787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23150.028.0848835
TCCGATC52050.027.4892023
TTCCGAT52400.027.1338582
CCGATCT52900.026.9624484
CGATCTA8150.025.6714945
CTTCCGA56350.025.6710011
CGATCTC18800.020.2234215
GAGACGT13350.019.8850428
GACGTGT11800.019.82791710
CGATCTT10750.018.4161975
ACGTGTG12800.017.57582911
GGAGACG16850.017.2232697
ACCGTCG3450.016.9543068
CGTCGTA3100.016.69136810
GTATATA9650.016.552241
GGGAGAC17400.016.4202676
TACCGTC3800.015.9847547
GATCTGG18600.015.8447046
AGACGTG15550.015.6249699
ATCTCGT2453.6379788E-1215.6102057
Produced by FastQC (version 0.11.2)