FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell95.35200000095b73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell95.35200000095b73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666846
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA52950.31766581915785863No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC34550.20727769691981143No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG30520.18310029840789133No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT28450.170681634656111No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA24610.1476441134933881No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT21690.1301259984425676No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT20160.12094698610429516No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA19130.11476765100075233No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC19090.11452767682197396No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC18660.11194795440010653No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC18640.11182796731071737No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTA17330.10396881295572596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG15350.027.113165
CCGTCGA507.141377E-527.010541
TTCCGAT35600.025.5930372
CCGATCT36600.024.9552384
TCCGATC36750.024.7921683
CGGTACC3750.024.01221545
CGATCTA6750.023.6630865
CTTCCGA41100.022.6614841
GTCGACG602.4606707E-422.510143
ACGTTTG1051.4484649E-619.28279511
GAGACGT11400.018.94458
GGGAGAC12250.018.9155046
ACGTTAT600.006516262418.7477251
CGATCTC10900.018.368785
TTAACGG4900.018.36842335
CGATCTT7600.018.3524865
GATCTCG3850.018.1141416
CGCGGTA5000.018.0080843
TCGACGG750.001107169218.0080844
CGTCGAC750.001107392318.0075442
Produced by FastQC (version 0.11.2)