FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell94.35200000095b4d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell94.35200000095b4d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2524460
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA47330.18748564049341246No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC26990.10691395387528423No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG25730.10192278744761256No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT25400.10061557719274618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23250.028.8358425
TTCCGAT50400.027.943652
TCCGATC50550.027.860733
CCGATCT51400.027.443774
CTTCCGA52500.026.9973161
CGATCTA7600.025.7540175
CGATCTT10950.021.5731965
GAGACGT11150.020.9844598
CTCGCGA550.003937832520.45453628
CGATCTC16550.019.1672785
GACGTGT11150.018.96672210
ACGTGTG11400.018.55078711
GGAGACG13400.018.3004197
GCGTATC750.001108367118.005744
GGGAGAC14650.017.1996546
CGGTACC3950.017.09503445
AGACGTG13100.016.315179
GTATTAG10500.016.2840941
CGTGTGC13450.016.22515112
TTAACGG4200.016.07174135
Produced by FastQC (version 0.11.2)