FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell93.35200000095b30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell93.35200000095b30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1503489
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25170.1674106029375672No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG16310.10848100651218598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT38700.026.6830482
TCCGATC40800.025.2545173
CTTCCGA41800.025.242381
CCGATCT40650.025.2370194
CGATCTG16800.024.9079155
CGATCTA7200.024.6847295
CCTAATA2200.021.4748612
CGATCTT7950.021.2240315
GAGACGT10900.020.8462838
AGACGTG11000.019.4296369
GGGAGAC12750.019.2331336
GACGTGT11250.018.79788810
CGTGTGC11800.018.68434112
ACGTGTG11350.018.23583811
GGAGACG13200.017.8957187
CGATCTC14900.017.8167925
GATCTGG8450.017.5719916
CGGTACC2500.017.11059845
GATCTCG4400.016.8731066
CGTGTGG1501.4253965E-716.49814617
Produced by FastQC (version 0.11.2)