FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell91.35200000095afb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell91.35200000095afb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104122
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24410.11601038342833733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG300.005147075730.00136438
CGATCTG18650.026.901515
TCCGATC39500.026.5993123
CCGATCT40200.026.0242084
TTCCGAT40650.025.9575042
CGATCTA7050.024.253545
CTTCCGA46650.023.3905661
CGATCTT7200.020.9359635
TTAGCGA550.00393874120.4535327
GAGACGT14100.020.2644718
GACGTGT13200.020.1121610
AGACGTG14150.019.0798729
GGGAGAC16450.019.0107636
ATCTGCG2400.018.7486257
GGAGACG16750.018.4016347
CGATCTC12400.018.3252685
ACGTGTG13150.018.3066411
CGCATCG3250.017.30642113
GATCTGC9600.017.108126
GTATATA7250.017.0677131
Produced by FastQC (version 0.11.2)