FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell90.35200000095ad1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell90.35200000095ad1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704279
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA18970.26935348065184395No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC12050.17109696583314282No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG10730.15235439364229234No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT9820.13943337796526659No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA8630.12253666515684836No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT8340.1184189788421918No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC7720.10961564947982264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA453.4903343E-529.998255
TTCCGAT16550.024.0620382
TCCGATC16700.023.7111913
CCGATCT16700.023.5764684
CGATCTG7000.023.4629155
CGATCTA3100.023.2244515
TAATACG802.9735475E-622.4986864
TCGTTAA500.002264513922.49868612
CGTATAT702.7022916E-522.4986868
GACGTGT6100.022.49868610
ATAGCGT602.467167E-422.4986864
GGGAGAC6900.022.4986866
ACGTGTG6050.022.31274611
CTTCCGA18500.022.012231
GGAGACG7200.021.8737247
GAGACGT6900.021.846558
AGACGTG6650.021.3145459
TCCGCCA855.030184E-621.17523618
CGATCTT2350.021.0626015
TTGCGGT2050.020.85244235
Produced by FastQC (version 0.11.2)