FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell9.352000000950a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell9.352000000950a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723420
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25930.1504566501491221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG16600.029.4097675
TCCGATC34900.029.2656233
TTCCGAT35150.029.1854822
CTTCCGA35350.028.8294351
CCGATCT35650.028.5245554
CGATCTA5950.025.333655
CGATCTC11100.023.1058435
CGTCGTA1950.020.76719510
TACCGTC2550.018.5275967
CGATCTT6900.018.2590795
TCTTCCG15050.018.0879295
TAAACCG750.001111259717.9982365
ACCGTCG2350.017.2323538
CGGTACT3150.017.14565338
GCTCTTC16200.016.664553
CGGTAGC1104.6435704E-516.36203410
ATACCGT2750.016.3620326
TGTACCG1803.918103E-916.2484075
GGTACTA3200.016.17451339
GATCTCG5150.016.1634646
Produced by FastQC (version 0.11.2)