FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell89.35200000095ab7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell89.35200000095ab7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006830
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA14800.14699601720250688No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC10160.10091077937685608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG401.5612008E-533.7473951
CGGGTAT450.001227001424.99806815
CGATCTG9550.024.5007255
TCCGATC20650.023.4243413
CCGATCT20700.023.1503874
TTCCGAT20750.023.09462
CTTCCGA21450.022.6555921
TAGGCGG500.002265432822.49826211
TCTGTCG654.2399374E-420.7676288
CGATCTA3200.020.3890515
TTAACGG1251.7662387E-819.80043635
GAGACGT6850.019.3780658
GACGTGT6350.019.13238110
TAGGCCG600.00651285218.748555
CGATCTT3150.018.5699945
AGACGTG6800.018.1971249
GGAGACG7450.018.1194067
TACTTCG750.001109928718.00039729
GGGAGAC7650.017.939796
ACGTGTG6550.017.86121411
Produced by FastQC (version 0.11.2)