FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell88.35200000095a9e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell88.35200000095a9e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440282
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8270.18783416083328414No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT7510.17057249671801256Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCT207.015525E-445.0169344
CTACATT207.0312497E-444.9964828
AGGTCTA207.0312497E-444.9964834
GAGCCGC1150.037.1752337
ACGCATG504.8512447E-836.01354240
GTGACCC250.002105334536.0012736
CTCAAGT250.002106508735.9971828
ACCTGCT250.002106508735.997187
TCGAACT250.002106508735.9971823
CGTGCAC1200.035.638443
ACGTTTT2050.035.119211
GTGATAT458.6581895E-735.0131744
GGCGGAT900.035.0131745
TCCTGAT458.6890395E-734.9972621
GCGGGCA653.6561687E-1034.62840742
GATACGT1050.034.283038
TTCGAAC401.5592239E-533.7473622
GGTATAC1300.032.88204235
AGACGTT8750.032.6545879
TGACGTC900.032.5048438
Produced by FastQC (version 0.11.2)