FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell87.35200000095a74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell87.35200000095a74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927850
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA14650.15789190063048986No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC10050.10831492159292991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG8400.026.7828275
TCCGATC19050.025.6271613
TTCCGAT19550.025.2018892
CCGATCT19500.025.1511364
CTTCCGA21100.023.883681
CGATCTT3200.023.2006245
CGATCTA3700.023.105625
TTAACGG1104.0508894E-922.535
GAGACGT4950.019.0888528
GGTACTA1901.8189894E-1218.95145439
TTGCGGT2150.018.8372135
CGGTACT1951.8189894E-1218.46452338
CGATCTC5850.018.4595495
GACGTGT5000.018.44801110
CGGTACC1406.4321284E-817.68810345
TAACGGC1406.463961E-817.68047536
ACGTGTG4900.017.44709811
GGGAGAC6300.017.141016
CCTTGCG2301.8189894E-1216.63043433
GGAGACG6350.016.2974557
Produced by FastQC (version 0.11.2)