FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell86.35200000095a5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell86.35200000095a5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347703
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA35440.26296595021306624No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC24330.18052938963555026No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG21340.15834349259443659No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT20150.14951365397272248No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA18880.14009021275459058No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT17540.13014736926459317No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC15230.11300709429303044No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT14260.10580966281146513No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA14230.10558706183780849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA4300.027.728815
TCCGATC26000.026.8232963
CCGATCT26400.026.5020964
TTCCGAT26750.026.071242
CGATCTG12800.025.133325
CTTCCGA28100.025.0598181
CGATCTT4200.024.6395245
GCCGTAA602.4669757E-422.50113137
TAACGCC1305.0931703E-1122.4969564
AACGCCG1358.367351E-1121.6637365
CGATCTC7700.020.7439485
CGTCGTA1552.3646862E-1120.31983210
CGCATCG1403.094101E-919.28310813
ACCGTCG1656.002665E-1119.0883278
GATCTCG3300.019.0883276
GAGACGT7800.018.4590448
GACGTTT1102.259485E-618.40660310
GACGTGT7100.018.06093810
GGCGTTA1253.8339203E-718.00023831
CGGTATA750.001111089617.99823424
Produced by FastQC (version 0.11.2)