FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell85.35200000095a31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell85.35200000095a31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2675687
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA64790.24214341961522404No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC47760.1784962142432953No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG39620.148074120777206No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT35360.13215297603942464No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA32590.12180049460194708No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT32330.12082878154283368No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC27840.10404804448352889No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT27670.10341269363718551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG22900.027.3125615
TTCCGAT52100.026.4713362
TCCGATC52450.026.3375873
CCGATCT52250.026.1800464
CTTCCGA56850.024.4970231
CGATCTT9000.022.4984765
CGATCTA9300.022.0146395
CGATCTC16350.021.053625
GACGTGT13500.020.165310
GAGACGT14300.019.8238338
ACGTGTG14000.019.12370511
CGTCGCG600.006511737618.75048328
GACACGC600.00651525118.7487318
CGGTACC4950.018.64102445
AGACGTG14850.018.4835979
GGAGACG17100.017.8935267
GGGAGAC17250.017.3466516
GATCTGG14700.017.2947486
GATCTCG6350.016.6524166
CGTGTGC16400.016.59948512
Produced by FastQC (version 0.11.2)