FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell84.35200000095a17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell84.35200000095a17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567984
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA31120.19847141297360177No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG20910.13335595261176134No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC18000.11479708976622212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTC507.142298E-527.0098440
CGGTAAG551.3686842E-424.54500228
CGATCTG13900.024.4388795
GACGTGT15850.023.13542410
AGACGTG15900.022.7796949
GAGACGT16600.022.7677618
CGATCTA6050.022.6826385
TTCCGAT32800.022.5653042
CGGTACC3100.022.5125145
GACGCGT802.968145E-622.50676339
TCCGATC33800.022.0308063
CCGATCT33550.021.9267544
ACGTGTG16300.021.66861511
GGAGACG17900.021.2399167
GGGAGAC18350.021.086846
ACGCGTG754.5910976E-521.00765240
TCCGCTC1301.2296368E-920.76686122
CTTCCGA37800.020.2946551
CGAGCGT1009.071937E-720.2470446
CGTGTGC17850.019.78702712
Produced by FastQC (version 0.11.2)