FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell83.352000000959fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell83.352000000959fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734793
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA46820.269888107687776No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC29580.17051025684332366No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG29450.1697608878984409No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT25020.14422470000743604No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT20960.12082133142109751No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA20830.12007196247621474No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT20130.11603689892684603No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC18580.10710211535324389No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC18170.10473872098861363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC29600.023.7897766
GGAGACG29600.023.1057247
CGATCTA7350.022.0385425
CGATCTG19000.021.7872265
GAGACGT31150.021.5948858
AGACGTG29950.020.732429
GACGTGT30050.020.66342710
GACGACT550.003929174520.46187242
CTGTGCG550.00393968620.4524369
CCGATCT45650.020.206024
ACGTGTG30300.019.97318611
TCCGATC46850.019.7364923
TTCCGAT46800.019.7095052
ACCGCCT3450.019.56320212
GACGTTT2500.018.89805210
GATACGT1202.4973997E-718.7507737
GAGCCGT1202.4973997E-718.7507737
CGTGTGC32850.018.62821612
TCGACCG851.2058426E-418.527518
CGGTACC3300.018.42046545
Produced by FastQC (version 0.11.2)