FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell82.352000000959c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell82.352000000959c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847694
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA45570.2466317474646776No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG27140.1468857938598058No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC24850.13449196674341096No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT23270.12594076724825648No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA20600.11149032253176122No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT20340.11008316312116617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1001.3460522E-924.74941825
GGGAGAC21300.023.5538716
GGAGACG21300.023.2370037
CGATCTG17300.023.1478675
TTCCGAT41550.022.4705752
GAGACGT20750.022.3350128
TCCGATC42050.022.1498813
CCGATCT41750.022.1473834
GCGACGT1358.367351E-1121.6679237
AGACGTG20900.021.0982699
GACGTGT20950.020.94052910
ACGTGTG21200.020.37522711
CGATCTC13300.019.6220055
CGATCTA7050.019.466055
CTTCCGA49600.019.3679331
CGTGTGC22750.018.98702412
GCGTATC600.006499624318.75615944
TTCGGTA600.00651385918.74905223
GGTCGAT901.8667859E-417.4995926
TGCTCGA901.8667859E-417.4995934
Produced by FastQC (version 0.11.2)