FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell81.352000000959ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell81.352000000959ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555417
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA28270.11062773707774504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC60300.025.0356143
CCGATCT60500.024.8412914
TTCCGAT61050.024.8386082
CGATCTG24600.024.5105345
CGATCTA11000.023.7256625
CTTCCGA68100.022.6306231
CGATCTT12250.022.0393225
GACGTGT22200.020.87696310
GAGACGT23000.020.7377248
GGAGACG24900.020.1492377
ACGTGTG22200.020.06620811
GGGAGAC26000.019.4698336
GTTACGT706.9898606E-419.2844053
CGATCTC19650.018.8918485
AGACGTG24700.018.6728239
CGTGTGC25900.017.80767312
CGGGTAT901.8677727E-417.49881415
ACGCGTG1156.776884E-515.65509440
GATCTCG8050.015.6511126
GGCGTTA3800.015.39881542
Produced by FastQC (version 0.11.2)