FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell80.35200000095982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell80.35200000095982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570214
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA38140.2428968280756636No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC22570.14373836941970966No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG20130.1281990862392005No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT19320.12304055370796592No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA17320.11030343634689285No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT16560.10546333174968507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA6050.026.0310335
CCGATCT31150.025.4233824
TCCGATC31200.025.2384193
TTCCGAT31350.024.974132
CGATCTG12950.024.3224325
CTTCCGA32600.024.0855481
TAGCACG702.705433E-522.498254
GAGACGT10000.020.698398
CGATCTC10200.020.0719685
GACGTGT9350.019.73108510
GGGAGAC11600.019.5889936
GGAGACG11300.019.3126567
TTACCGG706.9886865E-419.28421417
ATCTGCG1757.2759576E-1219.2842127
CGGTACT5350.018.92677538
TTGCGGT5500.018.81791535
ACGTCCG1202.4884503E-718.75690343
AGACGTG10500.017.99869
ACGTGTG9750.017.998611
CGATCTT6500.017.99865
Produced by FastQC (version 0.11.2)