FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell8.3520000009508b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell8.3520000009508b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2209187
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA58690.26566334131062697No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG39340.17807455864985627No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC35870.16236742294789894No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT30810.13946306944590928No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA26510.11999889552129359No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT26030.11782615052505742No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC23720.10736981523067084No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT22890.10361277700801244No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC22660.10257167003064928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG16700.030.3111865
TCCGATC38700.029.2992443
CCGATCT38950.029.168954
TTCCGAT38800.029.1077632
CGATCTA6750.028.3303555
CTTCCGA40800.027.901481
CGATCTT6850.023.9755825
GAGACGT8950.023.1260598
CGATCTC12950.021.8895915
GGAGACG10350.020.8673737
GGGAGAC11050.020.156256
GACGTGT9450.019.99789810
ACGTGTG9600.019.9197811
AGACGTG9950.019.4451929
CGTAGCG1757.2759576E-1219.28368615
GACGCGT951.3148394E-518.95052539
GCGGAAT1901.8189894E-1218.94537719
GATCTCG4900.018.8245536
GAATCGG1801.0913936E-1118.7480322
ACCGCCT5250.018.42663412
Produced by FastQC (version 0.11.2)