FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell79.35200000095968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell79.35200000095968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2408293
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25980.10787723918974976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG21450.026.7458825
TCCGATC50200.025.635183
CCGATCT50450.025.552744
TTCCGAT50550.025.54672
CTTCCGA54600.023.9813921
CGATCTA8650.023.148245
GATACGT602.4700785E-422.4980078
GAGACGT14150.020.9875418
CGATCTC17850.019.2840065
CGATCTT9600.018.9826955
GACGTGT13900.018.28974710
ACGTGTG14400.017.65468811
AGACGTG14950.017.155679
GGGAGAC18900.017.02236
GGAGACG18650.017.0092167
CGGTACC2251.8189894E-1217.00696845
CGGGTAT1451.812401E-615.51586615
GTATTAG9100.015.0810811
CGTCGAG900.003752019315.00209839
CGTGTGC17600.014.57257212
Produced by FastQC (version 0.11.2)