FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell78.3520000009594f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell78.3520000009594f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991431
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA12410.1251726040440535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA3600.028.7476485
TCCGATC20300.028.5922783
TTCCGAT20400.028.4521182
CCGATCT20500.028.2035874
CGATCTG8150.027.8811555
CTTCCGA22100.026.264821
CGATCTT4400.021.9868355
CGATCTC7500.021.2982585
CGGTACC600.00649212218.75887345
GAGACGT5050.017.8203248
GACGTGT5150.017.47429810
GATCTAC2800.016.873626
ACGTGTG5500.016.77135511
TATCGCC952.8184557E-416.57926433
GGGAGAC6400.016.5220856
GGAGACG6200.016.329317
GTTCTAG1855.6224962E-915.8095181
GCATTCG2151.5279511E-1015.6963922
CGTATTG2202.1645974E-1015.33965327
AGACGTG5650.015.1315049
Produced by FastQC (version 0.11.2)