FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell77.35200000095932.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell77.35200000095932.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042884
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27080.13255769784285354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG18900.026.424135
TCCGATC42750.026.3113673
CCGATCT43350.026.1028794
TTCCGAT43100.026.0977022
CGATCTA7450.024.4589775
CTTCCGA46850.024.0567871
TCGTCTA500.002265165322.50007233
CGATCTT9000.020.7465135
GAGACGT12300.019.7527778
CGATCTC13650.019.6120875
TTAACGG1951.8189894E-1218.4620535
GGCGTTA2300.017.61651642
GACGTGT12050.017.54891810
GGGAGAC15000.017.3970766
GTATTAG6800.017.202991
GGAGACG15250.016.8168477
ACGTGTG12700.016.65074511
GGTACTA3800.016.5842839
AGACGTG13400.016.2845769
CGGTACT3900.016.1582538
Produced by FastQC (version 0.11.2)