FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell76.35200000095918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell76.35200000095918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860753
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA31430.10986617859004257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC58350.028.1846183
TTCCGAT59150.027.879492
CCGATCT59550.027.541594
CTTCCGA62200.026.620921
CGATCTG26750.025.9883025
CGATCTT11600.025.0192875
CGATCTA10950.024.449845
CGATCTC19050.020.785525
GAGACGT14350.020.067878
GACGTGT13650.019.94324910
GGGAGAC17750.018.6321166
ACGTGTG14550.018.09114611
GTATATA8950.017.3444941
ATCTGCG3650.017.2587137
GGAGACG17850.017.1413067
GGCGTTA3100.016.7002142
AGACGTG16450.016.5486559
GTCGACG1104.6248737E-516.37045343
CGTCGAG1004.1659625E-415.75187939
TCGACGG1351.5761801E-515.00703644
Produced by FastQC (version 0.11.2)