FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell75.352000000958e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell75.352000000958e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451841
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25210.17364160400484627No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC15010.10338597683906157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG15150.025.2454785
CCGATCT35700.024.640864
TCCGATC36000.024.498013
TTCCGAT36150.024.396362
GGAGACG15500.023.5142217
GGGAGAC16300.023.1883036
TATCCGT500.002264149522.50127437
GAGACGT15400.022.4981758
CTTCCGA39950.022.41371
CGATCTA6250.022.3181925
GACGTGT15050.021.52649310
CGGGTAT955.5381133E-721.3140615
ACGTGTG14950.020.76754611
AGACGTG16100.020.122599
CGATCTT6550.019.9220495
CGATCTC11700.019.6137945
TATCCCG706.9884944E-419.2841515
TCGACAT600.00651176418.74977132
CGTCTAA600.00651435418.74847810
GTATTAG4300.018.3124681
Produced by FastQC (version 0.11.2)