FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell74.352000000958c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell74.352000000958c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491473
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA43280.17371249859019142No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC26110.10479744311899025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23850.028.1103485
TTCCGAT56450.027.0604782
TCCGATC57150.026.7290293
CCGATCT57450.026.5899854
CTTCCGA58800.026.2090681
CGATCTA9850.022.383515
CGATCTT10700.020.815645
CGATCTC20250.020.7756655
GAGACGT13400.020.6508878
CGATTAC1757.2759576E-1219.29459645
GATCTCG7300.018.491276
GACGTGT14050.018.41449710
ATCTCGC3450.018.2590127
GGAGACG15800.018.2259947
GGGAGAC16350.018.163296
GTATTAG10650.017.5334281
GGCGTTA2450.017.45561242
AGACGTG15950.016.7851289
GTATATA9550.016.4904691
GATCTGG15900.016.4134246
Produced by FastQC (version 0.11.2)