FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell73.352000000958ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell73.352000000958ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2264695
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG608.203291E-626.2470514
CGATCTG22300.025.019615
TCCGATC49850.024.2349913
CCGATCT49700.024.21764
TTCCGAT51100.023.7742372
CGATCTT8900.023.2558165
CTTCCGA54500.022.497471
GAGACGT15850.022.1426228
CGATCTA7750.020.6105885
ACGTGTG16200.020.41437311
GACGTGT16450.019.96736310
GGGAGAC19750.018.9092666
GGAGACG19600.018.7096337
TTCGCGA851.20104065E-418.53756144
AGACGTG18200.018.2946479
GTATTAG8800.017.8957181
GGCGTTA4300.017.27465442
CGATCTC16650.017.1602335
ATCTGCG2650.016.9792257
CGTGTGC20250.016.8869912
Produced by FastQC (version 0.11.2)