FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell72.35200000095890.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell72.35200000095890.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634496
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA36210.13744564425225925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG22950.026.5657025
TTCCGAT48350.026.3823222
TCCGATC48550.026.3199793
CCGATCT49200.025.926534
CTTCCGA51750.024.8663541
CGATCTA9200.024.209295
GAGACGT13350.019.0428478
CGATCTT8700.018.6186395
GACGTGT12850.018.5582710
CGATCTC15650.018.1130225
TCGAATT1751.382432E-1018.00485240
GGAGACG15000.017.998027
GGCGTTA3050.017.71137242
ACGTGTG13350.017.02059711
CGCTAGA5950.017.01816437
CCGCTAG5950.017.01784136
ATTCGTA6650.016.57775124
AGACGTG14300.016.5191619
GGTACTA4400.016.36742639
CGGTACT4400.016.36649338
Produced by FastQC (version 0.11.2)