FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell71.35200000095876.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell71.35200000095876.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660860
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19820.2999122355718306No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG13370.20231213872832368No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC10810.1635747359501256No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT9300.1407257210301728No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT8390.1269557848863602No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT7700.11651484429379899No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA7480.1131858487425476No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC7210.1091002632932845No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC7190.10879762733407984No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC7150.10819235541567049No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATA7080.10713312955845415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG207.033146E-444.99735338
TACGCGT250.002106287736.00060739
ACGCGTG352.8163075E-432.14826240
TATAACG352.8200616E-432.1409654
TAACGTA300.005147175429.9982366
ATAACGT406.1654695E-428.1233445
CGATCTA2400.027.18595
GTACTAG507.156738E-526.9984111
CGATCTG5100.025.1455785
CCGATCT13250.024.1117884
TCCGATC13450.023.753253
TTCCGAT13450.023.753252
GGAGACG6750.022.9986467
CGCGCTT702.69724E-522.50378441
AGCTGCG602.4669524E-422.49867620
CGGCTCG500.00226437322.49867636
GGGAGAC7200.021.8737136
GAGACGT7000.021.5344498
AGACGTG7050.021.062599
CGACCTT550.00393613620.45334225
Produced by FastQC (version 0.11.2)