FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell70.3520000009585d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell70.3520000009585d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879164
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA89330.3102636737608556No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG52280.1815804865579036No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC50080.17393937962547462No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT44340.15400303699268258No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA39200.13615063261418942No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT38510.13375410362174578No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT33580.11663107763225714No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC32410.11256739803637444No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC31280.10864264765744502No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA30940.10746174931334233No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC29600.10280762054540832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG300.005145179630.00399638
CGATCTG23600.025.8340235
TTCCGAT56050.024.2431222
TCCGATC56900.023.9600433
CCGATCT56450.023.9521924
TCGACGG702.6969654E-522.5084744
CGTTAAG500.002265051522.5006529
CTTCCGA62300.022.2443581
GGGAGAC22450.022.0465726
GGAGACG22300.021.7913257
GAGACGT21150.021.6997418
CGGTACC5550.021.09011545
TCGAGTA1401.364242E-1020.89890741
GACGCGT908.245122E-620.00370639
AGACGTG21100.019.9385669
CGATCTT12000.019.8728145
CGATCTC16900.019.8350965
GACGTGT21250.019.79782310
CGTCGAC807.548203E-519.69422742
CGATCTA9600.019.2166375
Produced by FastQC (version 0.11.2)