FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell7.3520000009507e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell7.3520000009507e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486385
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA41350.16630570084681173No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC27560.11084365454263921No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG27220.10947620742564003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT38650.029.5699332
TCCGATC38700.029.4154623
CCGATCT40000.028.4594614
CTTCCGA40400.028.3447441
CGATCTG18250.028.229865
CGATCTA7150.025.486795
CGATCTC13050.022.41145
CGATCTT7700.020.1601835
GGCGTTA2750.017.18827842
CGGTACT5300.016.55949638
CACGATT2057.4578566E-1116.4686140
TCTTCCG17350.016.3383125
GATCTCG6250.016.1982696
GTCGACG850.00255986215.88832543
CCGTCGA850.002560549415.88768641
ATCTGCG3050.015.4901497
CCGGTAG2051.3205863E-915.3642129
TCACGTA2800.015.272675540
GATCTGC11800.015.2526086
GCGTTAT3250.015.23649643
Produced by FastQC (version 0.11.2)