FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell69.35200000095826.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell69.35200000095826.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810545
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA9170.11313375568290471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG6400.029.5289845
CCGATCT16050.027.7548794
TCCGATC16150.027.5830213
TTCCGAT16250.027.5517312
CTTCCGA17550.025.8954491
CGATCTA2600.022.4982725
CGATCTT4050.021.109495
ACTTAGG550.003937302620.4529742
CGATCTC5650.019.9099755
CGCGAAG706.9846463E-419.28423322
GACGTGT3700.018.84990310
CCCGCGA851.2045934E-418.52798820
ACGACGG1102.255967E-618.4076776
ATCTGCG1102.255967E-618.4076777
GATCTGT3450.018.2594686
CGGCATG750.001107197518.00639344
CTCGTTA1153.4486493E-617.60734411
CCGCTAG1551.0888471E-817.41909236
CGTTATT1053.1011237E-517.14894744
GGCGTTA1053.1044114E-517.1468342
Produced by FastQC (version 0.11.2)