FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell68.3520000009580c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell68.3520000009580c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999479
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA13850.13857219611417548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAA300.0051361230.01291543
CGATCTG9350.027.4298615
TCCGATC20350.027.0838433
TTCCGAT20550.026.8202532
CCGATCT20900.026.4800724
CTTCCGA21650.025.4575611
CGATCTA3050.024.341345
GAGACGT5050.022.7200438
GTACCGT500.00225940522.5085641
GTATTAT1601.8189894E-1221.090161
GACGTGT5150.020.53151510
CGATCTT4600.020.051945
GGGAGAC6150.019.3879156
ATAGCGT1403.090463E-919.28346
ACGTGTG5100.018.9683111
ACAATCG600.006503148518.75337837
AGACGTG5550.018.646419
TAGCGTA1454.776666E-918.6184547
CGATCTC7100.018.3780775
CGTCGTA1253.8368125E-717.99783910
Produced by FastQC (version 0.11.2)