FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell67.352000000957e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell67.352000000957e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940626
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA12160.12927561007244112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG9000.027.7480035
TTCCGAT19000.027.588652
TCCGATC19400.027.0198143
CCGATCT19550.026.8124964
CGATCTA3300.025.9072255
CTTCCGA20900.025.5111621
TCCGATG500.002264554622.49957722
ATCTGCG1204.5110937E-1022.4983797
CGTCTAA550.003931598720.4585140
TAGCCCG550.00393765720.45307210
CGATCTT3750.020.398535
TATCGCC1051.4452598E-619.28534933
CGATCTC6050.018.9655745
GACGTGT5400.018.7486510
GTGCGTA600.00651234618.7486515
CGCCTAT1102.2554996E-618.40874336
TCTATCG1102.2554996E-618.40874331
TCGATTA750.00110739118.00636142
ACGTGTG5450.017.75101511
GAGACGT6350.017.715268
Produced by FastQC (version 0.11.2)