HT5LJBCXY_l01n02_mock_cell66.352000000957c7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HT5LJBCXY_l01n02_mock_cell66.352000000957c7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269531
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1750	0.6492759645458221	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT	499	0.18513640360478015	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACG	410	0.15211608312216404	No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	387	0.14358274187384754	No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	383	0.14209868252631422	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCT	352	0.13059722258293108	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCT	279	0.10351313949044823	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCTTC	20	7.028233E-4	44.994064	25
CATTAGG	20	7.034662E-4	44.985714	1
GAGGGCG	35	6.220269E-6	38.580654	33
GGGCTGC	35	6.2440995E-6	38.55918	6
GCTACGT	65	9.094947E-12	38.064835	8
AGTCCTC	25	0.0020998681	36.0153	43
ATACGCG	50	4.8370566E-8	36.0153	38
CTATACT	25	0.002101783	36.008614	28
AGGCGGC	45	8.638308E-7	35.014874	34
TGCTCCC	45	8.638308E-7	35.014874	45
TTGGGCG	45	8.650877E-7	35.008373	33
AGACTTT	45	8.6886575E-7	34.988888	9
TCGCCTG	65	3.6379788E-10	34.623665	29
GGCAGCC	40	1.5516312E-5	33.764343	35
ATACGCA	40	1.5516312E-5	33.764343	38
CACCCGT	40	1.5575599E-5	33.74555	20
ATTAGGG	40	1.5595406E-5	33.73929	2
CTACGTG	40	1.5595406E-5	33.73929	9
TGCACAC	55	1.12540874E-7	32.716885	16
TGTGGAC	35	2.8076748E-4	32.156517	43