FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell65.352000000957ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell65.352000000957ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296471
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT29050.026.795112
TCCGATC29350.026.5978763
CCGATCT29550.026.2666034
CGATCTA4950.025.9066935
CTTCCGA30500.025.5950051
CGATCTG14150.025.121355
CGATCTT5150.023.5900485
CTATGCG807.5648466E-519.68719728
ACGACGT706.9866655E-419.28467425
CGATCTC9450.018.8077835
ACGATTA600.006501373418.75477441
TATGCGC851.2047346E-418.52912529
GAGACGT8800.018.4073878
GACGTGT8600.018.31225810
GGGAGAC9750.018.2290826
ACGTGTG8600.017.7890511
GGAGACG9650.017.7185677
TTAACGG1153.4473233E-617.60978335
CCCGCGA1853.074092E-1017.02610820
CGTACGC800.001712506316.8727873
Produced by FastQC (version 0.11.2)