FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell64.35200000095791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell64.35200000095791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808066
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC352.821849E-432.138571
CGATCTA3150.026.4250475
ATGCACG709.3126437E-725.7108546
TCCGATC18900.024.9966643
CCGATCT19000.024.983514
TTCCGAT19250.024.5421812
CGATCTT3500.023.7825435
CTTCCGA21050.023.0848061
CGATCTG8350.022.9011365
CCGCTAG1601.8189894E-1221.09615936
GGGAGAC6850.021.0190946
CGCTAGA1651.8189894E-1220.4568837
GGAGACG7150.020.1371757
CATTCGT1900.020.12889323
ATTCGTA1800.019.99733424
GAGACGT6900.019.888658
GCATTCG1703.6379788E-1219.85029422
GACGTGT6650.019.62144310
ACGTGTG6850.019.37697811
CGTAATC600.006509223518.74981931
Produced by FastQC (version 0.11.2)