FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell63.35200000095777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell63.35200000095777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468809
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA45000.3063706717483349No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC25470.17340580020955754No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG24740.16843578709008455No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT22090.1503939586426826No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT20990.1429048977777233No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA18450.1256119754168173No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT16760.11410605463337982No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC16530.11254016008888834No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTA16080.10947645337140499No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC15960.10865946491340943No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA15360.10457452262343163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCGT450.001226089725.00219337
GGGAGAC18150.024.6673896
CGATCTG15950.024.4023765
GGAGACG17900.024.3834657
GCGACGT952.1143933E-823.6862937
TTCCGAT35250.023.6150722
AATACGC1152.6557245E-1023.4763265
GAGACGT17700.023.3879018
CCGATCT36350.023.1480244
CGATCTA5800.022.8860445
TCCGATC36950.022.6503663
GACGTCG602.464223E-422.50504139
AGACGTG17450.021.9179639
GACGTGT17250.021.65038910
ATACGCC1056.11617E-821.4268056
CTTCCGA40050.021.2903791
CGATCTT6250.021.2382475
ACGTGTG16900.021.0337711
CGGTACT4950.020.9123538
CCTTGCG5000.020.70040733
Produced by FastQC (version 0.11.2)