FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell62.3520000009575e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell62.3520000009575e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289232
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG13550.024.9054535
TCCGATC29550.024.0577623
TTCCGAT29700.023.8605122
CCGATCT30200.023.3164834
CTTCCGA31350.022.96351
CGATCTT5650.021.9006355
ACTACGC855.0370145E-621.1745178
GTCTAAC550.00393978220.4518661
CGATCTA4850.019.482745
GAGACGT9800.019.2839368
TACGCAA600.006514382518.7482710
ATCTGCG1709.276846E-1118.5277027
GACGTGT9700.018.32305310
GGAGACG11600.017.4552867
GATCTGA7150.017.3060976
GGGAGAC12000.017.0609266
CGTTTGC1205.18054E-616.8727912
CCCTCGA2159.094947E-1216.74264326
TATACTG3400.016.5425915
GTATTAG4000.016.3103621
Produced by FastQC (version 0.11.2)