FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell61.35200000095734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell61.35200000095734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3003707
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA70930.23614154110237784No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC46980.15640673341307923No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT39640.13197026207949045No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG38960.12970639280062934No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT37660.12537840741457137No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA34470.1147581971210907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT58500.027.304522
CGATCTG26500.027.082215
TCCGATC58900.027.0808943
CTTCCGA60350.026.728461
CCGATCT60150.026.555524
CGATCTA10850.025.5043745
CGATCTT11150.022.800425
CGGGTAT1104.0636223E-922.49776315
GACGTGT13800.021.19354410
GAGACGT15200.019.9815648
ACGTGTG14600.019.41587611
GTATTAG14500.018.6185281
AGACGTG15500.018.433659
CGATCTC19200.018.279435
CGGTACC5800.018.24035845
GGGAGAC18550.017.8284156
GGAGACG18300.017.7031567
TCGAGTA1406.4641426E-817.68387841
ATCTGCG3900.017.305977
CGTGTGC17750.017.1106912
Produced by FastQC (version 0.11.2)