FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell60.3520000009570d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell60.3520000009570d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657874
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC47450.026.4080373
CGATCTA9550.026.1478965
CCGATCT48250.025.9701844
TTCCGAT48450.025.9094142
CGATCTG21600.025.6211435
CTTCCGA53150.023.9568821
CGATCTT8950.020.3600645
GACGTGT15750.019.42564810
GAGACGT17900.019.103278
CGATCTC14900.019.0239835
AGACGTG16400.018.7929049
GGGAGAC20000.018.6721886
GGAGACG20800.017.4132467
ACGTGTG16750.017.3257511
CGCGGAT952.8126108E-416.585542
GCATTCG7750.015.67623922
GTATTAT5550.014.9977431
GTATTAA8350.014.8181291
GATCTGC11650.014.6759016
CGGTCCG4350.014.48684739
Produced by FastQC (version 0.11.2)