FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell6.35200000095055.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell6.35200000095055.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2675962
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA43900.1640531517263698No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC27770.10377576363192002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA44150.031.4927711
TTCCGAT44250.031.014272
TCCGATC44650.030.7364253
CGATCTA7550.030.3947285
CCGATCT45550.030.079734
CGATCTG20850.029.9974085
CGATCTT9050.022.6223555
CGATCTC14250.022.5769985
CGTAGCG1950.019.6136915
GAATCGG1851.6370905E-1118.24166922
CGGGTAT1752.6975613E-916.71284315
ACGACCG1257.640025E-616.198617
GGGGTTA8050.015.9303026
TAGACCG850.002567838215.8809825
GGTACTA6100.015.86313939
CGGTACT6000.015.75187838
GCGGAAT2151.546141E-1015.69631919
TCTTCCG16700.015.6273945
GATCTGG14200.015.5268296
ATCTGCG2950.015.252927
Produced by FastQC (version 0.11.2)