FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell59.352000000956e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell59.352000000956e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857718
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA22200.2588263275342245No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG14100.16438969451498045No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC12350.1439867182453907No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT10530.1227676229250173No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT9560.11145854464987327No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC9280.1081940684467389No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA9260.10796089157508645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC12950.023.106376
GGAGACG13000.022.6713757
CCGTAAT500.002265901322.49699814
GCGACGT754.60148E-520.99964737
GAGACGT13250.020.7154258
ATCGCCT1201.1037628E-820.62585434
GAATCGG550.00393744920.45300922
CGATCTA3650.020.3409395
GTATTAA2000.020.2472971
CGATCTG9850.019.8716015
AGACGTG13200.019.60089
TCCGATC22150.019.5007843
CCGATCT22450.019.3404054
CGGGAGA2100.019.2842665
TTCCGAT22450.019.1399862
GATCGGG951.314725E-518.94815432
GACGTGT13300.018.94594410
CGCAGTG600.006512006318.7485916
ATCGCTA600.006512006318.7485910
CGCTATC600.006514198618.74749812
Produced by FastQC (version 0.11.2)