FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell58.352000000956c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell58.352000000956c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326947
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19120.14409015582385734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG450.001227565224.99681914
CGATCTG12250.024.9773525
TCCGATC26550.024.3189373
TTCCGAT27550.023.5995372
CCGATCT27300.023.5693174
CTTCCGA29900.021.9712751
GACGTGT9450.020.2362810
CGATCTA4350.020.1706075
GGGAGAC10500.020.1410526
GAGACGT10050.019.9235888
CGATCTT5000.019.7982255
CGGTACT1850.019.4606538
GGAGACG10600.019.3143087
ACGTGTG9700.019.2508511
GTATTAG5150.019.2215791
ATACCGT1801.0913936E-1118.7483226
AGACGTG9850.018.5008819
CGGTAAA750.001111013917.99838611
TACCGTC1902.3646862E-1117.7615667
ATATCGC901.86723E-417.4984326
Produced by FastQC (version 0.11.2)