FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell57.352000000956af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell57.352000000956af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301588
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24040.10444962347735563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG21250.025.5150345
TCCGATC48750.025.0128253
CCGATCT48550.024.9768474
TTCCGAT49800.024.6209742
CGATCTA9250.024.5650525
CTTCCGA53450.023.109021
GAGACGT17400.021.7219148
GGAGACG18950.021.3698447
GACGTGT16850.021.09576210
CGATCTT11000.020.2479295
GGGAGAC20750.020.166616
ACGTGTG16750.020.1471911
ATCTGCG3000.019.4980037
AGACGTG18250.019.3541839
TATACGG951.3179302E-518.945435
GTATTAG8350.017.7833841
CGTGTGC20150.017.52922212
TTAACGG2850.017.36890635
GATCTAC6000.016.498316
TTAGACT2600.016.4406244
Produced by FastQC (version 0.11.2)