FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell56.35200000095685.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell56.35200000095685.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771284
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA3000.029.2474295
TTCCGAT13700.028.0814762
TCCGATC13900.027.6774233
CTTCCGA13900.027.5155661
CCGATCT14100.027.1252754
CGATCTG6150.027.0707935
GATCTAA1401.364242E-1020.8910226
GTATTAA2300.020.5416721
TTACGTG550.00393738420.4527520
CGATCTT2100.020.3553545
CGATCTC4500.019.9982435
GTATATA2050.019.7543621
TGTGCGG706.984747E-419.2840210
GGCGTTA951.3122379E-518.95184742
GTATTAG1951.8189894E-1218.4599151
TAATACC1406.474329E-817.6770174
GACGTGT2600.017.3061710
TCTATAC1053.1126227E-517.1413523
CGGTAAT800.00170967816.87570635
GAGACGT3350.016.789578
Produced by FastQC (version 0.11.2)