FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell55.3520000009566b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell55.3520000009566b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799605
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA10720.13406619518387203No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC300.005150076529.9955417
TCCGATC14100.026.0067713
CCGATCT14200.025.9820524
CGATCTG6100.025.4470375
CGATCTA2600.025.0924225
TTCCGAT14800.025.080732
GGCGTTA1101.5097612E-1024.55562642
CTTCCGA14950.024.5281261
CGATCTT2400.023.4340155
GAGACGT4700.021.539358
CGCGCAA1156.7684596E-921.51988621
TACTCGT550.00392749620.4617439
CGCCGTA550.003937469320.45278425
TATATAG1452.1827873E-1020.1694152
GGGAGAC5700.019.7339066
GACGTGT4600.019.56230710
GTATAGC1151.5963269E-719.5623071
GGAGACG5350.019.3429187
GTACTCG706.976158E-419.28767238
ATTTCGT706.984973E-419.28405423
Produced by FastQC (version 0.11.2)