FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell54.35200000095642.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell54.35200000095642.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845125
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA42370.1489214006414481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA10300.028.1770065
TCCGATC61350.027.833123
TTCCGAT61300.027.7824212
CCGATCT62350.027.4232834
CGATCTG28100.027.3818055
CTTCCGA64900.026.3106611
ACGCGTG1056.0956154E-821.43525340
CGATCTC19900.021.2543135
CGATCTT11350.020.2183935
GAGACGT19000.018.8272068
GACGTGT18100.018.39608610
ACGTGTG18700.018.16676911
GGGAGAC21650.017.7697186
GGAGACG21500.017.3704857
GACGCGT1205.166621E-616.87907639
AGACGTG20500.016.7911289
GTATATA9400.016.0354671
CGTGTGC22850.015.75318812
AGCGCGT900.003749707715.00362239
CGTTTCG900.00375146215.00256738
Produced by FastQC (version 0.11.2)