FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l01n02_mock_cell53.35200000095635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l01n02_mock_cell53.35200000095635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362622
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8950.027.6513675
TCCGATC48350.027.360743
TTCCGAT49250.026.9064272
CGATCTG20950.026.8474455
CCGATCT49300.026.8335064
CTTCCGA51200.025.8377291
CGATCTT9600.021.7950925
CGATCTC16650.019.1876185
GATCTAG4600.018.0963466
TTAACGG1459.682299E-817.06865335
GACGTGT12000.017.06110410
GAGACGT12300.016.644988
ATCTGCG2850.016.577597
TACCGTC3000.016.498657
GTATATA7250.016.1366121
ACGTGTG11700.015.767940511
GGGAGAC14750.015.4055196
GCATTCG6600.014.9990922
TTCGACT900.003757000414.99908925
GATCTCG7250.014.8953336
Produced by FastQC (version 0.11.2)